细胞周期调控对大鼠全脑缺血后海马迟发性神经元死亡的影响

目的观察细胞周期调控对大鼠全脑缺血再灌流后海马区迟发性神经元死亡(delayed neuronal death,DND)以及星形胶质细胞的活化、增殖的影响.方法建立大鼠短暂性全脑缺血再灌流模型,利用尼氏染色、TUNEL、免疫组织化学方法观察再灌流后细胞周期素依赖的蛋白激酶(cyclin depedent kinase, CDK)抑制剂Olomoucine对海马DND以及星形胶质细胞活化增殖的影响.结果全脑缺血再灌流后3d、7d、30d海马神经元明显脱失,部分CA1、CA2区神经元凋亡;星形胶质细胞数目增多,GFAP表达上调,应用Olomoucine后TUNEL阳性神经元数目明显减少,幸存神经元数目增加;星形胶质细胞数目无明显增多,GFAP表达明显下调.结论 CDK抑制剂Olomoucine可有效抑制大鼠全脑缺血后海马神经元DND以及星形胶质细胞活化增殖.

EFFECT OF CELL CYCLE REGULATION ON DELAYED NEURONAL DEATH IN RAT HIPPOCAMPUS AFTER GLOBAL ISCHEMIA

Objective To explore the effect of cell cycle regulation on the delayed neuronal death and the activation and proliferation of astrocytes in rat hippocampus after global ischemia.Methods Arat model of transient global ischemia was used to observe the effect of Olomoucine (cyclin dependent kinase inhibitor) on delayed neuronal death and astrocytic activation and proliferation in the hippocampus by Nissel dyeing, TUNEL and immunohistochemical techniques.Results Hippocampal neurons were significantly lost 3, 7 and 30 days after transient global ischemia, part of the neurons in CA1 and CA2 regions underwent apoptosis, while the number of astrocytes and the expression of GFAP were increased. With Olomoucine, the mumber of TUNEL-positive neurons and the expression of GFAP were decreased, whereas the number of survival neurons increased. Conclusion Olomoucine can effectively inhibit the delayed neuronal death and the activation and proliferation of astrocytes in the hippocampws after global ischemia.