High affinity binding of the large T protein of polyoma virus to a genomic mouse DNA sequence |
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| Authors: | C Galup P Léopold L Trejo-Avila P el Baze M Rassoulzadegan P Gaudray F Cuzin |
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| Institution: | Unité 273 de l'INSERM, Université de Nice, France. |
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| Abstract: | We purified a fragment of mouse DNA to which the large T protein of polyoma virus was bound in chromatin prepared from transformed mouse cells. This sequence, which is not repeated to a measurable extent within the mouse genome, does not show any significant homology to the viral ori region, except in a short region, which comprises a sequence related to the consensus for recognition by large T proteins ((A,T)GPuGGC). This region of pCG4 was confirmed by in vitro binding assays to be essential for T antigen binding. |
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