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| 牛肠激酶催化亚基工程菌的构建 | |
| 引用本文： | 张春香,罗学刚,童锦禄,蒋声海,符玉梅,杨道文.牛肠激酶催化亚基工程菌的构建[J].现代生物医学进展,2011,11(3):489-492. |
| 作者姓名： | 张春香 罗学刚 童锦禄 蒋声海 符玉梅 杨道文 |
| 作者单位： | 1. 南京医科大学附属江宁医院药剂科,江苏,南京211100 2. 天津科技大学生物工程学院,天津,300457 3. 上海市仁济医院消化科,上海,200127 |
| 摘 要： | 目的:构建牛肠激酶催化亚基工程菌。方法:将带有牛肠激酶催化亚基(bEKL)的结构基因进行克隆,将克隆产物纯化后,构建bEKL克隆质粒,后构建bEKL表达质粒,并将表达质粒制导入备感受态细胞,进行蛋白表达。结果:琼脂糖电泳验证牛肠激酶催化亚基基因已经成功地插入表达载体,序列与预期设计一致。结论:表达质粒构建成功。 |
| 关 键 词： | 牛肠激酶催化亚基 工程菌 构建 |
Building Bovine Enterokinase Catalytic Subunit Engineering Bacteria |
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| ZHANG Chun-xiang,LUO Xue-gang,TONG Jin-lu,JIANG Sheng-hai,FU Yu-mei,YANG Dao-wen.Building Bovine Enterokinase Catalytic Subunit Engineering Bacteria[J].Progress in Modern Biomedicine,2011,11(3):489-492. | |
| Authors: | ZHANG Chun-xiang LUO Xue-gang TONG Jin-lu JIANG Sheng-hai FU Yu-mei YANG Dao-wen |
| Institution: | ZHANG Chun-xiang1,LUO Xue-gang2,TONG Jin-lu3,JIANG Sheng-hai1,FU Yu-mei1,YANG Dao-wen1(1 Dept.of Pharmacy,Nanjing Jiangning Hospital attached to NanJing Medical University,Nanjing 211100,China,2 Bioengineering Institute of TianJin Technological University,Tianjin 300457,3 Dept.of Digest,Shanghai RenJi Hospital,Shanghai 200127) |
| Abstract: | Objective:To build a Bovine Enterokinase Catalytic Subunit Engineering Bacteria.Methods:Clone the structural gene of Enterokinase Catalytic Subunit,and construct bEKL clonic plasmid.Then construct bEKL expressive plasmid,and translate the expressive plasmid into competent cell.Results:The agarose electrophoresis revealed that Bovine Enterokinase Catalytic Subunit gene had been successfully inserted expressive plasmid and the sequence was same with primary design.Conclusion:The expressive plasmids was succes... |
| Keywords: | Bovine enterokinase catalytic subunit Engineering bacteria Building |
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