l-Ribulose production by an Escherichia coli harboring l-arabinose isomerase from Bacillus licheniformis |
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Authors: | Ye-Wang Zhang Marimuthu Jeya Jung-Kul Lee |
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Institution: | (1) Department of Chemical Engineering, Konkuk University, Seoul, 143-701, Korea;(2) Institute of SK-KU Biomaterials, Konkuk University, Seoul, 143-701, Korea;(3) School of Pharmacy, Jiangsu University, Zhenjiang, 212013, PR China; |
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Abstract: | Recombinant Escherichia coli harboring the l-arabinose isomerase (BLAI) from Bacillus licheniformis was used as a biocatalyst to produce l-ribulose in the presence of borate. Effects of substrate concentration, the borate to l-arabinose ratio, pH, and temperature on the conversion of l-arabinose to l-ribulose were investigated. l-Ribulose production was efficient when pH was higher than 9 and temperature was higher than 50 °C. Borate addition to the
reaction mixture was essential for high conversion of l-arabinose to l-ribulose as it resulted in an equilibrium shift in favor of the product. Under the optimal conditions determined by response
surface methodology, the E. coli harboring BLAI produced 375 g l−1 L-ribulose from 500 g l−1
l-arabinose at a reaction time of 60 min, corresponding to a conversion yield of 75% and productivity of 375 g l−1 h−1. When the resting recombinant E. coli cells were recycled, 85% of the yield was obtained even after seven cycles of reuse. The productivity and final concentration
of l-ribulose obtained in the present study were the highest yet reported. |
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