Oxidation-reduction midpoint potentials of the molybdenum center in spinach NADH:nitrate reductase |
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| Authors: | M J Barber B A Notton L P Solomonson |
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| Institution: | 1. Department of Biochemistry, University of South Florida, College of Medicine, Tampa, FL 33612, USA;2. University of Bristol, Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, England |
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| Abstract: | Oxidation-reduction midpoint potentials for the molybdenum center in assimilatory NADH:nitrate reductase isolated from spinach (Spinacia oleracea) have been determined at pH 7.0 in the presence of dye mediators using EPR spectroscopy to monitor formation of Mo(V). Values for the Mo(VI)/Mo(V) and Mo(V)/Mo(IV) couples were determined to be -8 and -42 mV, respectively. |
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