Overexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture |
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| Authors: | Xinghui?Zhao Junwei?Guo Yingqun?Yu Shaoqiong?Yi Ting?Yu Ling?Fu Lihua?Hou Email author" target="_blank">Wei?ChenEmail author |
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| Institution: | (1) State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, People’s Republic of China;(2) Department of Anaesthesia, Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie Street, Fengtai District, Beijing, 100071, People’s Republic of China; |
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| Abstract: | To optimize Chinese Hamster Ovary (CHO) cell culture to recombinant protein therapeutic production, we stably overexpressed
survivin and cyclin D1 in three CHO DG44-derived cell lines. The modifications conferred increases of 56–94% in S-phase fractions
and decreases of 33–43% in early-stage apoptosis fractions. Clone 6.3, which expressed the highest levels of survivin and
cyclin D1, reached significantly greater cell densities in suspension (2.7 × 106 cells/ml) following serum deprivation. Nude mice inoculated with the modified cells showed no tumorigenesis suggesting that
the CHO DG44-derived cell lines are viable candidates for biopharmaceutical production. |
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