不同生态区罂粟种质的遗传多样性ISSR分析

为了揭示罂粟种质的遗传多样性,利用ISSR分子标记进行遗传多样性研究,从100条随机引物中筛选出9条多态性引物对所有样品扩增,共检测到109个等位变异位点,引物等位位点数在9～16之间,平均每条引物有12个等位变异,其中,引物807的等位位点最多,为16个。引物等位位点多态性信息量PIC值为0.77～0.92。其中引物807的PIC值最大(0.92),引物847的PIC值最小(0.77),遗传相似系数在-0.452～0.981之间,且明显聚为2个类群;亲缘关系和地理分布呈一定的相关性,但没有形成明显的地理变异模式;本研究将为罂粟种质资源科学有效利用提供理论依据。

ISSR Genetic Diversity of Poppy Germplasms in Different Ecological Zones

In order to reveal the genetic diversity of poppy in different ecological zones. Based on the molecular markers of 100 inter-simple sequence repeat ( ISSR), poppy and relatives from 12 different areas were amplified and analyzed with optimizing conditions for the primers. The results showed that a total of 109 allelic variations were detected, between 7-16 loci for each primer. Among them, primer 807 had the most alleles of 16. The value of allelic polymorphism information content (PIC) ranged from 0.77-0.91, which the Maximum is 807 (0.92) and the minimum is 847 (0.77). The results showed that the genetic similarity among them ranged from -0.45 to 0.98, and these 12 germplasms were classified into 2 groups.The research will provide basis theoretical for scientific and effective utilization poppy germplasms.