Biochemical and Autoradiographic Study of Cerebral Protein Synthesis with [18F]- and [14C]Fluorophenylalanine |
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Authors: | W. Bodsch,H. H. Coenen&dagger ,G. Stocklin&dagger ,&dagger K. Takahashi,K.-A. Hossmann |
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Affiliation: | Max-Planck-Institut für Neurologische Forschung, Abteilung für Experimentelle Neurologie, K?ln, F.R.G. |
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Abstract: | Fluorine-18-labeled ortho or para isomers of L-fluorophenylalanine were used in double-label experiments together with L-[3H]phenylalanine for amino acid incorporation into cerebral proteins of Mongolian gerbil brain. It was demonstrated by qualitative regional comparison of the 18F and 3H autoradiographic images that L-p-[18F]fluorophenylalanine is incorporated into proteins and exhibits a regional cerebral protein synthesis pattern. To a minor extent, L-p-fluorophenyl[3-14C]alanine and L-o-[18F]fluorophenylalanine are hydroxylated in vivo to form labeled tyrosine or tyrosine analogues that are incorporated into cerebral proteins as well. The advantage and validity of the application of L-p-[18F]fluorophenylalanine with positron emission tomography for noninvasive studies of cerebral protein synthesis in humans are evaluated on the basis of an experimental animal approach. |
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Keywords: | Protein synthesis Fluorophenylalanine Phenylalanine Fluorine-18 Autoradiography. |
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