The characteristics of arginine transport by rat cerebellar and cortical synaptosomes |
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Authors: | Christian R Aldridge Keith J Collard |
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Institution: | (1) Physiology Unit, School of Molecular and Medical Biosciences, University of Wales College of Cardiff, P.O. Box 911, CF1 3US Cardiff, UK;(2) Present address: Department of Medical Sciences, St. Loye's School of Occupational Therapy, Millbrook House, Millbrook Lane, Topsham Road, EX2 6ES Exeter, UK |
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Abstract: | The uptake ofl-3H]arginine into synaptosomes prepared from rat cerebellum and cortex occurred by a high-affinity carrier-mediated process.
The uptake of arginine appeared to be potentiated by removal of extracellular Na+, inhibited by high levels of extracellular K+, but not by depolarization with veratridine or 4-amino pyridine. The effect of Na+ removal or K+ elevation did not seem to be due to changes in intracellular Ca2+ or pH. In both brain regions, uptake was significantly inhibited byl-arginine,l-lysine,l-ornithine, andl-homoarginine, but not byd-arginine norl-citrulline. Uptake was also inhibited by NG-monomethyl-l-arginine acetate, but not by NG-nitro-l-arginine methyl ester nor NG-nitro-l-arginine except in the cortex at a concentration of 1 mM. The results indicate that the carrier system operating in synaptosomes
showed many of the characteristics of the ubiquitous y+ system seen in many other tissues, although its apparent sensitivity to variations in extracellular Na+ was unusual. |
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Keywords: | l-Arginine" target="_blank">l-Arginine uptake synaptosomes cerebellum cortex nitric oxide |
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