Characterization of a newly established feline lymphoma-derived cell line (BKD) lacking T and B cell surface markers |
| |
Authors: | Robert W Engelman Katsuhiko Machida Ross E Longley Wing T Liu Liem Q Trang Robert A Good Noorbibi K Day |
| |
Institution: | (1) Cancer Research Program, Oklahoma Medical Research Foundation, 73104 Oklahoma City, Oklahoma;(2) All Children's Hospital, 801 6th Street South, 33701 St. Petersburg, Florida;(3) Department of Pathology, Animal Resources, Oklahoma University Health Sciences Center, 73104 Oklahoma City, Oklahoma |
| |
Abstract: | Summary A new feline lymphoma-derived cell line, designated BKD, was isolated from an anterior mediastinal tumor. Cells of this line
were characterized as lymphoid based on morphology, the lack of intracellular esterase and peroxidase activity, and absence
of phagocytic function. In contrast with other established feline lymphoma-derived cell lines, cells of the BKD line lack
characteristics of both feline T-cells and B-cells in that they neither form rosettes with guinea pig erythrocytes nor have
demonstrable surface or cytoplasmic immunoglobulin. Approximately one third of BKD cells form EAC rosettes, a significant
number of rosette forming cells (p=0.0001) when compared to background sheep E-rosetting activity. In addition, a consistently titratable level of interleukin-2-like
activity was produced when BKD cells were coincubated with concanavalin A and phorbol-12-myristate-13-acetate. Chromosome
analysis showed that a majority of BKD cells are diploid. This new cell line has been continuously replicating in culture
for over one year and produces feline leukemia virus as demonstrated by several analyses.
This research was aided by grants AI-22360 and CA-34103 from the National Institutes of Health and by grant IM-298 from the
American Cancer Society. |
| |
Keywords: | feline leukemia virus cell line |
本文献已被 SpringerLink 等数据库收录! |
|