Intermediate filament heterogeneity in normal and hypercholesterolemic rabbit vascular smooth muscle cells |
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| Authors: | L Molony P O Hagen F H Schachat |
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| Institution: | 1. Department of Anatomy, Duke University Medical Center, Durham, NC 27710, USA;2. Department of Biochemistry Duke University Medical Center, Durham, NC 27710, USA;3. Department of and Surgery, Duke University Medical Center, Durham, NC 27710, USA;1. School of Dentistry, Health Science Center, Shenzhen University, Shenzhen, 518015, China;2. School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, 518015, China;3. School of Basic Medicine, Health Science Center, Shenzhen University, Shenzhen, 518015, China;4. Department of Ecology and Evoluitonary Biology, University of Connecticut, Storrs, CT, 06269-3043, USA;5. Department of Dentistry - Regenerative Biomaterials, Radboudumc, Nijmegen, 6525EX, the Netherlands;6. Research Institute for Medical Innovation, Radboudumc, 6500HB, Nijmegen, the Netherlands;7. State Key Laboratory of Fine Chemicals, School of Bioengineering, Dalian University of Technology, Dalian, 116023, China;8. Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology, Dalian, 116024, China;1. The Ciccarone Center for the Prevention of Cardiovascular Disease, Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;2. Welch Center for Prevention, Epidemiology, and Clinical Research, Johns Hopkins University, Baltimore, Maryland, USA;3. Division of Cardiovascular Prevention and Wellness, Department of Cardiology, Houston Methodist DeBakey Heart and Vascular Center, Houston, Texas, USA;4. Division of General Internal Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, USA;1. School of Electronic Science & Engineering, Southeast University, Nanjing, Jiangsu 210096, China;2. College of Mechanical Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China;1. School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China;2. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;1. Department of Chemical & Biological Engineering, Korea National University of Transportation, Chungju 27469, Republic of Korea;2. Chemical Industry Institute, Korea National University of Transportation, Chungju 27469, Republic of Korea;3. Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of Korea;4. Department of Biological Sciences, College of Health Sciences, Wonkwang University, Iksan, Chunbuk 570-749, Republic of Korea;5. Department of Green Bio Engineering, Korea National University of Transportation, Chungju 27469, Republic of Korea |
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| Abstract: | We have examined the intermediate filament (IF) protein content of vascular smooth muscle (SM) cells from several arteries and veins in rabbits and quantitated the changes which occur in SM cell expression of these proteins in response to cholesterol feeding. Cells from control rabbit arteries expressed 30% of their IF protein as desmin, while veins expressed 50% as desmin. During development of diet-induced atherosclerosis, morphological changes in arterial SM cells in the intima correlate with changes in IF expression. There is a significant increase in total IF protein content, vimentin increased differentially in thoracic aorta and desmin in pulmonary artery. In abdominal aorta both increase equally. Cholesterol feeding also resulted in changes in the expression of subspecies of desmin, vimentin, and actin in the thoracic arch. Although cholesterol feeding did not produce obvious morphological changes in the veins examined, venous SM IF protein expression was also altered. In the vena cava of cholesterol-fed rabbits there was an increase in vimentin expression without the parallel increase in desmin that occurred in the arterial system. These studies show that cholesterol feeding of rabbits induces measurable changes in the amounts of IF proteins in both arterial atherosclerotic lesions and venous SM cells. |
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