穿心莲内酯衍生物抗H2O2诱导胰岛RIN-mβ细胞凋亡的蛋白组学研究

采用蛋白组学方法筛选穿心莲内酯衍生物(AL-1)抗过氧化氢诱导胰岛RIN-mβ细胞凋亡的差异蛋白质分子并探讨其作用分子机制.结果显示:AL-1浓度依赖性地提高H₂O₂处理的胰岛RIN-mβ细胞的存活率.经蛋白组学研究分析,成功地鉴定了18个与凋亡、应激等相关的蛋白,包括Prohibitin、Shmt2、RhoGDP-dissociationinhibitor-1、Galectin-1、Cyt b5、Hsps等;与对照组(H₂O₂)相比,处理组(AL-1+H₂O₂)中,有9个表达上调的蛋白和9个表达下调的蛋白.AL-1通过调控与细胞凋亡、应激等相关的蛋白发挥其抗H₂O₂诱导的凋亡作用.

Proteomic study of andrographolide analogue mediated protection on cell apoptosis induced by H2O2 in RIN-m beta cells

To screen the differential proteins invovled in andrographolide analogue (AL-1) against apoptosis induced by hedrogen peroxide (H₂O₂) in Rin-m beta cells and investigate its molecular mechanism with proteomics. The results showed that:The survival rate of cells treated by H₂O₂ were increased by AL-1 in a dose-dependent manner. With proteomics analysis, 18 proteins related to apoptosis, stress were successfully identified, such as Prohibitin, Shmt2, Rho GDP-dissociation inhibitor-1, Galectin-1, Cyt b5, Hsps, and so on. Among them, 9 proteins were found to be up-regulated and 9 down-regulated in the AL-1 treated group compared with control (H₂O₂) group. AL-1 protected Rin-m beta cells against apoptosis induced by H₂O₂ via regulating proteins associated with apoptosis, stress.