Determination of the branch location of extra N-acetyllactosamine units in sialo N-linked tetraantennary oligosaccharides |
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| Authors: | C H Hokke J P Kamerling G W van Dedem J F Vliegenthart |
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| Institution: | Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands. |
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| Abstract: | An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-acetyl-beta-glucosaminidase, yielding products which could be analysed by 1H-NMR spectroscopy, thereby giving conclusive data about the location of the extra units in the intact structures. |
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