Immunochemical studies on xanthine dehydrogenase of soybean root nodules |
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| Authors: | J. Nguyen L. Machal J. Vidal C. Perrot-Rechenmann P. Gadal |
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| Affiliation: | (1) Institut de Physiologie Végétale, Centre National de la Recherche Scientifique, F-91190 Gif-sur-Yvette, France;(2) Physiologie Végétale Moléculaire, UA 1128 CNRS, Université Paris-Sud, Bât. 430, F-91405 Orsay Cedex, France;(3) Structure et Métabolisme des Plantes, UA 1128 CNRS, Université Paris-Sud, Bât. 430, F-91405 Orsay Cedex, France |
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| Abstract: | Xanthine dehydrogenase (XDH, EC 1.2.1.37) was purified from root nodules of soybean (Glycine max) and used to prepare a polyclonal rabbit antiserum. Monospecificity of this antiserum was ascertained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipate. During root nodule development of soybean, only one form of XDH was detected on an immunological basis. Titration of XDH by immunoelectrophoresis showed that a remarkable increase in the amount of XDH occurred between two and four weeks after inoculation, in parallel with the increase in enzyme activity. Localization of XDH by immunofluorescence indicated that the enzyme was present exclusively in uninfected cells where it appeared to be associated with discrete organellelsAbbreviations IgG immunoglobulin G - SDS-PAGE sodium dodecyl sulfate — polyacrylamide gel electrophoresis - XDH xanthine dehydrogenase |
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| Keywords: | Glycine (xanthine dehydrogenase) Immunocytochemistry Polyclonal antibody Root nodule Xanthine dehydrogenase |
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