Ontogenetic expression of chromogranin A and its derived peptides, WE-14 and pancreastatin, in the rat neuroendocrine system |
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Authors: | Sharon C Barkatullah W J Curry Colin F Johnston John C Hutton Keith D Buchanan |
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Institution: | (1) Department of Medicine, School of Clinical Medicine, The Queen’s University of Belfast, Grosvenor Road, Belfast BT12 6BJ, UK; Tel. +44 1232 263226; fax +44 1232 329899; e-mail: J.W.Curry@QUB.AC.UK, GB;(2) Department of Clinical Biochemistry, Addenbrooke’s Hospital, Cambridge, UK, GB |
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Abstract: | The ontogenetic expression of chromogranin A (CgA) and its derived peptides, WE-14 and pancreastatin (PST), was studied in
the rat neuroendocrine system employing immunohistochemical analysis of fetal and neonatal specimens from 12.5-day embryos
(E12.5), to 42-day postnatal (P42) rats. CgA immunostaining was first detected in endocrine cells of the pancreas, stomach,
intestine, adrenal gland and thyroid at E13.5, E14.5, E15.5, E15.5 and E18.5, respectively. PST-like immunoreactivity was
detected in endocrine cells of the pancreas at E13.5, stomach, intestine at E15.5, adrenal gland at E17.5 and thyroid at E18.5.
WE-14 immunoreactivity was first observed in the immature pancreas at E15.5, mucosal cells of the stomach at E15.5, scattered
chromaffin cells in the immature adrenal gland and mucosal cells of the intestine at E17.5 and thyroid parafollicular cells
at E18.5. These data confirm that the translation of the CgA gene is regulated differentially in various neuroendocrine tissues
and, moreover, suggests that the posttranslational processing of the molecule is developmentally controlled.
Accepted: 18 October 1996 |
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