1-Cys peroxiredoxin knock-out mice express mRNA but not protein for a highly related intronless gene |
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Authors: | Mo Yiqun Feinstein Sheldon I Manevich Yefim Zhang Qunwei Lu Lu Ho Ye-Shih Fisher Aron B |
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Institution: | Institute for Environmental Medicine, University of Pennsylvania Medical Center, One John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104-6068, USA. |
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Abstract: | 1-Cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin family with a single conserved cysteine, is a unique antioxidant enzyme. We have generated mice in which the 1-cysPrx gene has been inactivated; they are viable and fertile. Mice have a highly related intronless gene (1-cysPrx-P1, GenBank accession number AF085220) with the same length of open reading frame (224 aa) as 1-cysPrx but located on a different chromosome. Since the product of this gene possibly could mimic 1-cysPrx function, we compared expression of 1-cysPrx and 1-cysPrx-P1 in mouse tissues by real-time polymerase chain reaction and Western blot. 1-cysPrx mRNA and protein were expressed in all mouse tissues that were examined with the highest expression level in lung. 1-cysPrx-P1 mRNA was expressed only in testis. In the 1-cysPrx 'knock-out' mouse, 1-cysPrx-P1 mRNA expression level was similar to the wild type but protein expression was not detected. Thus, mouse 1-cysPrx-P1 is an mRNA-expressed pseudogene that does not result in detectable protein in vivo. |
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Keywords: | 1-Cys peroxiredoxin 1-Cys peroxiredoxin related intronless gene 1 Pseudogene Knock-out mouse |
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