Identification and Determination of 1-Methylimidazole-4-Acetic Acid in Human Cerebrospinal Fluid by Gas Chromatography-Mass Spectrometry |
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Authors: | Carl-Gunnar Swahn Göran Sedvall |
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Institution: | Laboratory of Clinical Neurochemistry, Department of Psychiatry, Karolinska sjukhuset, Karolinska institutet, Stockholm, Sweden |
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Abstract: | Abstract: A method for the quantification of 1-methylimidazole-4-acetic acid in human CSF was developed. Methylimidazole-acetic acid was identified and quantitated in CSF. The method involves concentration of the compound on a cation exchanger, extraction of the methyl ester with ethyl acetate, and preparation of a heptafluorobutyryl derivate of the methyl ester, which is finally purified by chromatography on silica gel and quantitated by gas chromatography-mass spectrometry with the deuterated analogue as internal standard. The coefficient of variation at 1 ng/ml was 13%. The limit of sensitivity was about 0.2 ng/ml. The concentration of methylimidazole-acetic acid in lumbar CSF from healthy volunteers was below 1 ng/ml. Ventricular CSF contained higher concentrations than lumbar fluid. The existence of a rostrocaudal concentration gradient was established. There was a correlation between the concentration of methylimidazole-acetic acid and tele-methylhistamine in CSF. The concentration of methylimidazole-acetic acid in lumbar CSF from schizophrenic patients, patients with subarachnoidal haemorrhage, or patients with rheumatic disease was in the range of that in healthy volunteers. |
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Keywords: | Methylimidazole-acetic acid CSF Gas chromatography Mass spectrometry schizophrenia Rheumatoid arthritis Subarachnoidal haemorrhage |
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