Digitization of DGGE (denaturing gradient gel electrophoresis) profile and cluster analysis of microbial communities |
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| Authors: | Tong Zhang Herbert HP Fang |
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| Institution: | 1. Centre for Environmental Engineering Research, Department of Civil Engineering, The University of Hong Kong, Pofkulam Road, Hong Kong SAR, China
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| Abstract: | Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA profiles were objectively digitized using an image analyzer; the individual microbial species in a community can thus be precisely quantified. The similarity between various microbial communities was compared to the digitized DGGE profiles using the cluster analyses technique. The microbial community in a biofilm was considerably different from that in suspended sludge obtained from the same system. |
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