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Mitochondrial genetic damage induced in yeast by a photoactivated furocoumarin in combination with ethidium bromide or ultraviolet light
Authors:M. H. Juliani   S. Hixon  E. Moustacchi
Affiliation:(1) Fondation Curie, Institut du Radium, Biologie, Université Paris-Sud, Bâtiment 110, 91405 Orsay, France
Abstract:Summary Ethidium bromide (EB) and ultraviolet light (UV) in combination are known to produce a synergistic induction of ldquopetiterdquo mutants in yeast. Two other agents were combined with EB, 3-Carbethoxypsoralene (3 CPs) activated by 365 nm light or gamma rays. EB in combination with 3 CPs also resulted in an enhanced production of ldquopetiterdquo mutants. After the photoaddition of 3 CPs in exponential phase cells, recovery of the ldquopetiterdquo mutation during dark liquid holding was inhibited by the presence of EB producing an enhanced number of ldquopetiterdquo mutants. The behavior of mitochondrial antibiotic resistance markers after individual and combined treatments with EB and 3 CPs indicates a random loss of markers after EB and a preferential loss of a certain region for the 3 CPs photoaddition. The combination of the two agents leads to an additivity of total drug marker losses rather than a synergistic loss. The combination of EB with gamma rays produced no enhancement in ldquopetiterdquo induction. A combination of UV and 3 CPs showed a synergistic interaction for ldquopetiterdquo induction. These results indicate that the three agents, EB, UV and 3 CPs photoaddition may share a common repair step for mitochondrial lesions.
Keywords:
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