Analysis and comparison of a set of expressed sequence tags of the parthenogenetic water flea <Emphasis Type="Italic">Daphnia carinata</Emphasis> |
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Authors: | Xiaoqian Xu Shuhui Song Qun Wang Fen Qin Kan Liu Xiaowei Zhang Songnian Hu Yunlong Zhao |
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Institution: | (1) Department of Life Science, East China Normal University, 200062 Shanghai, People’s Republic of China;(2) Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029 Beijing, People’s Republic of China |
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Abstract: | The water flea Daphnia carinata (D. carinata) reproduces both sexually and parthenogenetically, yet little is known about the genes involved in these processes. To further
clarify the reproductive biology of Daphnia and elucidate their unique mechanism of reproductive transformation, we have generated and characterized an expressed sequence
tag (EST) data set from D. carinata. A set of 1,495 clusters were generated from sequencing 3,072 randomly chosen clones from a parthenogenetic, juvenile water
flea cDNA library. The nucleic acid and deduced amino acid sequences were compared with known GenBank sequences. Functional
annotation found that 959 clusters showed significant homology with known genes involved in a broad range of activities, including
metabolism, translation, development and reproduction, as well as genes involved in sensing environmental factors. We speculate
that genes involved in development and reproduction, along with genes that allow the organism to sense changes in the environment,
play important roles in the process of parthenogenetic reproduction and could be markers of the early steps of sexual differentiation.
Additionally, 86% of the D. Carinata unique sequences could be stringently mapped to the D. pulex genome, of which 125 mapped to intergenic and intronic regions on the current assembly. Our results provide practical insight
into crustacean reproductive biology, in addition to establishing a new animal model for reproductive and developmental biology.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Xiaoqian Xu and Shuhui Song contributed equally to this work.
Nucleotide sequence data reported are available in the GenBank databases under the accession numbers: GD269049−GD272045. |
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Keywords: | Daphnia carinata Parthenogenetic ESTs Chemosensory proteins |
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