A Paecilomyces fumosoroseus mutant over-producing chitinase displays enhanced virulence against Bemisia tabaci |
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Authors: | Ismael Hernández-Torres Magdalena Iracheta Luis J. Galán-Wong Carlos Hernández Juan Contreras Mark Jackson Benito Pereyra-Alférez |
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Affiliation: | (1) Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, México;(2) ARS-USDA, Peoria, IL, USA |
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Abstract: | A Paecilomyces fumosoroseus strain was mutagenized by u.v. Among 200 colonies, one mutant (M84), showed a large and stable chitin hydrolysis-halo. Glucose consumption and biomass production were similar for M84 and the parental strain. Chitinase was inducible by chitin and repressed by glucose in both strains but, when they were grown on minimal medium plus colloidal chitin as sole carbon source, the parental and M84 strains yielded 198 and 690 mol N-acetylglucosamine, respectively. This results indicate that the mutant strain synthesized a chitinase with a higher activity. Bioassays against Bemisia tabaci nymph, showed that M84 incited a 2-fold higher incidence of disease compared to the parental strain. |
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Keywords: | Bemisia tabaci biological control chitinases Paecilomyces fumosoroseus pest whitefly |
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