Human cytomegalovirus UL84 protein contains two nuclear export signals and shuttles between the nucleus and the cytoplasm |
| |
Authors: | Lischka Peter Rauh Claudia Mueller Regina Stamminger Thomas |
| |
Affiliation: | Institut für Klinische und Molekulare Virologie, Universit?t Erlangen-Nürnberg, Schlossgarten 4, D-91054 Erlangen, Germany. |
| |
Abstract: | Previous studies defined pUL84 of human cytomegalovirus as an essential regulatory protein with nuclear localization that was proposed to act during initiation of viral-DNA synthesis. Recently, we demonstrated that a complex domain of 282 amino acids within pUL84 functions as a nonconventional nuclear localization signal. Sequence inspection of this domain revealed the presence of motifs with homology to leucine-rich nuclear export signals. Here, we report the identification of two functional, autonomous nuclear export signals and show that pUL84 acts as a CRM-1-dependent nucleocytoplasmic shuttling protein. This suggests an unexpected cytoplasmic role for this essential viral regulatory protein. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|