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Optimal fixation conditions for the immunoperoxidase identification of human J chain from tissue sections
Authors:K. Käyhkö
Affiliation:(1) Fourth Department of Medicine, Helsinki University, Central Hospital, Unioninkatu 38, SF-00170 Helsinki, Finland
Abstract:Summary J chain can be used as a marker of plasmablasts and plasma cells at an earlier stage than intracellular immunoglobulin. Immunoperoxidase techniques were used to study optimal fixation conditions for the preservation of human J chain antigenicity in paraffin-embedded tissue sections. The most constantly positive staining for J chain combined with good morphological integrity was obtained with Bouin's fluid for 1.5 h at 20°C. All other fixatives studied showed less consistent staining results.Studies supported by the Sigrid Jesélius Foundation
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