Isolation, growth and identification of colony-forming cells with erythroid, myeloid, dendritic cell and NK-cell potential from human fetal liver |
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Authors: | Marcus O Muench David L Suskind Alicia Bárcena |
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Affiliation: | (1) Department of Laboratory Medicine, University of California at San Francisco, 3rd & Parnassus Ave., Room U-440, 94143-0793 San Francisco, CA |
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Abstract: | The study of hematopoietic stem cells (HSCs) and the process by which they differentiate into committed progenitors has been
hampered by the lack of in vitro clonal assays that can support erythroid, myeloid and lymphoid differentiation. We describe a method for the isolation from
human fetal liver of highly purified candidate HSCs and progenitors based on the phenotypes CD38−CD34++ and CD38+CD34++, respectively. We also describe a method for the growth of colony-forming cells (CFCs) from these cell populations, under
defined culture conditions, that supports the differentiation of erythroid, CD14/CD15+ myeloid, CD1a+ dendritic cell and CD56+ NK cell lineages. Flow cytometric analyses of individual colonies demonstrate that CFCs with erythroid, myeloid and lymphoid
potential are distributed among both the CD38− and CD38+ populations of CD34++ progenitors.
Published: June 11, 2002. |
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Keywords: | Indexing terms" target="_blank">Indexing terms fetal tissue hematopoietic stem cells cell differentiation natural killer cells dendritic cells |
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