The spring energy budget of the algal mat community in a Crimean hypersaline lake determined by microcalorimetry

The energy contents (standing stock) of the floating mat formed by the green alga Cladophora sivaschensis and the energy transfers through it were quantified for a shallow hypersaline lake (at Cape Khersones, Crimea, Ukraine) during the spring months. Appropriate direct calorimetric techniques were applied to: (i) measure the heat energy dissipated by the mat community and by the free bacterioplankton in the water column below it; and (ii) differentiate between the heat flows by the heterotrophic and the phototrophic components of the community. It was shown that Cladophora biomass reached a peak of 579.5 g C m^–2, contributing more than 99.6% of the total mat community. Throughout the spring, the total bacterial energy transfer (6 to 23 mW m^–2) was as little as 1.1 to 2.6% of the total heat dissipated by the microplankton community. The rest of the estimated heat energy (584 to 1488 mW m^–2) was associated with Cladophora metabolism. In the spring community: (i) the rate of biomass accumulation in the lake photic layer significantly exceeded its heterotrophic mineralisation; (ii) the efficiency of the microbial loop was too low to process even a minor part of the accumulated organic matter. The microcalorimetric technique was shown to be a highly promising approach for further studies of natural microbial mats and biofilms, biological systems with complex metabolism that involves not only aerobic processes but also anaerobic catabolism under local hypoxic/microxic conditions.