| 发光酶基因标记的荧光假单胞菌X16L2在小麦根圈的定殖动态 |
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| 引用本文： | 王平,胡正嘉,李阜棣.发光酶基因标记的荧光假单胞菌X16L2在小麦根圈的定殖动态[J].微生物学报,2000,40(2):150-154. |
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| 作者姓名： | 王平 胡正嘉 李阜棣 |
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| 作者单位： | 华中农业大学农业微生物农业部重点实验室,武汉,430070 |
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| 基金项目： | 国家自然科学基金(39270022)和国际科学基金(C／2362-1)资助 |
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| 摘 要： | 在土壤微宇宙系统内及田间土壤中，采用发不酶基因标记检测技术研究了荧光假单胞菌（Ｐｓｅｕｄｏｍｏｎａｓ ｆｌｕｏｒｅｓｃｅｎｓ，简称Ｐｆ）Ｘ１６Ｌ２在小麦根圈的定殖动态，研究结果表明：在不同灭菌灰潮土微宇宙中，Ｐｆ．Ｘ１６Ｌ２在小麦播种后３６ｄ定殖密度可达最高水平（３．２＊１０＾４ｃｆｕ．ｇ＾－１根），然后开始下降，最后保持在一个相对稳定的较低水平（约３．２＊１０＾２ｃｆｕ．ｇ＾－１根））。在田间条
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| 关 键 词： | 荧光假单胞菌 发光酶标记基因 根圈定殖 小麦 |
| 文章编号： | 0001-6209(2000)02-0150-54 |
BOOT COLONIZATION OF WHEAT BY lux-AB GENES MARKED PSEUDOMONAS FLUORESCENS X16L2 |
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| Wang Ping,Hu Zhengjia,Li Fudi.BOOT COLONIZATION OF WHEAT BY lux-AB GENES MARKED PSEUDOMONAS FLUORESCENS X16L2[J].Acta Microbiologica Sinica,2000,40(2):150-154. |
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| Authors: | Wang Ping Hu Zhengjia Li Fudi |
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| Institution: | Wang Ping
,Hu Zhengjia
,Li Fudi
;(Key lab of Agricutural microbiology, Ministry of Agricuture, Huazhong Agricultural University, Wuhan 430070) |
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| Abstract: | Colonization density of Pseudomonas fluorescens Xl6L2 marked with luxAB genes in wheat rhizosphere in asepsis rhizobox-Calcareous chao soil microcosms reached the maximum(4.60 log cfu.g-1 root) 3 days after seeds coated with Pf.Xl6L2 sown, then declined to a relatively stable lower level(2.45 log cfu.g-1 root) in 0-2 cm root segment. Dynamics of colonization of Pf.Xl6L2 in wheat rhizosphere under field conditions was the same as in microcosms, Pf.Xl6L2 could move to the place of 10 cm of root from seeds under field conditions, distance of horizontal movement of Pf.Xl6L2 in field soil was not over 40 cm during 125 days of plant growth. |
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| Keywords: | Pseudornonas fluorescens luxAB marker genes Root colonization Wheat Molecular ecology |
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