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The preparation of cultured rabies virus and the production of antiserum for human use
Authors:C A Consales  J G Valentini  A Albas  R M Mendonca  R M Fuches  M A Soares  C A Pereira
Affiliation:1. Laboratorio de Imunologia Viral, Instituto Butantan, Av. Vital Brasil, 1500 CP65, 05504 São Paulo, Brazil;2. Servico de Virologia, Instituto Butantan, Brazil;1. Department of Environmental Sciences and Engineering, Government College University Faisalabad, Faisalabad 38000, Pakistan;2. Department of Biological Sciences and Technology, China Medical University, Taichung 40402, Taiwan;3. Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad 38040, Pakistan;4. Department of Environmental Sciences, The University of Lahore, Lahore 54590, Pakistan;5. University of Wuppertal, School of Architecture and Civil Engineering, Institute of Foundation Engineering, Water and Waste Management, Laboratory of Soil and Groundwater Management, Pauluskirchstraße 7, 42285 Wuppertal, Germany;6. Department of Environment, Energy and Geoinformatics, Sejong University, 98 Gunja-Dong, Guangjin-Gu, Seoul, Republic of Korea
Abstract:In this paper we describe a methodology for the preparation of the Pasteur strain of fixed rabies virus in BHK-21 clone 13 cells and also its use for the production of antisera in horses. The methodology showed here is simple, rapid, facilitates the attainment of high protective titers, and the antisera produced are of high quality.
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