| 一株甲基对硫磷降解菌——米曲霉JMUPMD-2的分离与鉴定 |
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| 引用本文： | 解顺昌,倪辉,蔡薇,曹樱,李利君,肖安风,黄高凌,杜凤君,蔡慧农. 一株甲基对硫磷降解菌——米曲霉JMUPMD-2的分离与鉴定[J]. 微生物学通报, 2011, 38(7): 1007-1013 |
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| 作者姓名： | 解顺昌 倪辉 蔡薇 曹樱 李利君 肖安风 黄高凌 杜凤君 蔡慧农 |
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| 作者单位： | 1. 集美大学生物工程学院,福建厦门,361021
2. 厦门市出入境检验检疫局,福建厦门,361026 |
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| 基金项目： | 集美大学中青年创新团队专项基金(No. 2008A002) |
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| 摘 要： | 对一株新分离的、能以甲基对硫磷为唯一磷源生长的菌株——JMUPMD-2利用形态观察和rDNA ITS序列分析对JMUPMD-2进行鉴定; 用气相色谱法检测培养过程中甲基对硫磷浓度的变化, 确定甲基对硫磷降解速率。该菌株的rDNA ITS序列与米曲霉 (Aspergillus oryzae)的同源性为99%, 菌落形态和显微形态都与米曲霉特征相符, 因此鉴定为米曲霉; 以350 μg/L甲基对硫磷为唯一磷源和350 μg/L甲基对硫磷及1 g/L K2HPO4组成的混合磷源分别培养该菌株, 200 h后的分解量分别为189 μg/L及28 μg/L。该菌株的胞内提取液具有明显的甲基对硫磷降解酶活性。
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| 关 键 词： | 甲基对硫磷 生物降解 ITS序列 米曲霉 菌种鉴定 |
Isolation and identification of a parathion-methyl degrading fungus-Aspergillus oryzae JMUPMD-2 |
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| XIE Shun-Chang,NI Hui,CAI Wei,CAO Ying,LI Li-Jun,XIAO An-Feng,HUANG Gao-Ling,DU Feng-Jun and CAI Hui-Nong. Isolation and identification of a parathion-methyl degrading fungus-Aspergillus oryzae JMUPMD-2[J]. Microbiology China, 2011, 38(7): 1007-1013 |
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| Authors: | XIE Shun-Chang NI Hui CAI Wei CAO Ying LI Li-Jun XIAO An-Feng HUANG Gao-Ling DU Feng-Jun CAI Hui-Nong |
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| Affiliation: | 1. Bio-tech Engineering College, Jimei University, Xiamen, Fujian 361021, China;1. Bio-tech Engineering College, Jimei University, Xiamen, Fujian 361021, China;1. Bio-tech Engineering College, Jimei University, Xiamen, Fujian 361021, China;1. Bio-tech Engineering College, Jimei University, Xiamen, Fujian 361021, China;1. Bio-tech Engineering College, Jimei University, Xiamen, Fujian 361021, China;1. Bio-tech Engineering College, Jimei University, Xiamen, Fujian 361021, China;1. Bio-tech Engineering College, Jimei University, Xiamen, Fujian 361021, China;2. Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen, Fujian 361026, China;1. Bio-tech Engineering College, Jimei University, Xiamen, Fujian 361021, China |
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| Abstract: | A newly isolated fungus JMUPMD-2,capable of growing with parathion-methyl as the sole phosphorus source,was identified based on morphological charaterisitics and rDNA ITS sequences analysis,and its capability of degrading parathion-methyl was investigated by determining the changes of parathion-methyl concentration in the broth of the isolate.JMUPMD-2's colonic and microscopic morphologies were identical with and its sequences of rDNA ITS was 99% similar to Apspergillus oryzae.Consequently,it was identified... |
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| Keywords: | Parathion-methyl Biodegradation ITS sequence Aspergillus oryzae Strain identification |
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