| 神经肽注射液多肽成份的分离及鉴定 |
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| 引用本文： | 牛美娟,韩庆宏.神经肽注射液多肽成份的分离及鉴定[J].生物技术,1996,6(3):28-29. |
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| 作者姓名： | 牛美娟 韩庆宏 |
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| 作者单位： | 哈尔滨医科大学微生物教研室，哈尔滨医科大学生化教研室 |
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| 摘 要： | 本实验采用ＳｅｐｈａｄｅｘＧ２５及ＡＣＡ凝胶柱层析技术，对神经肽注射液中多肽成份进行过柱分离。将过柱后收集的不同洗脱峰的溶液通过紫外扫描，茚三酮显色，微量双缩脲试验及薄层层析等技术进行鉴定。此外还用ＳＤＳ—ＰＡＧＥ检测该注射液纯度及分子量。结果表明神经肽注射液含有一种主要多肽成份及大量小分子肽类，其主要多肽成份的分子量为１４，０００Ｄ。
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| 关 键 词： | 神经肽 分离鉴定 SDS—PAGE |
The Isolation and Identification of Peptide Composition from Neuropeptide Injectio |
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| Niu Meijuan et al.The Isolation and Identification of Peptide Composition from Neuropeptide Injectio[J].Biotechnology,1996,6(3):28-29. |
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| Authors: | Niu Meijuan |
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| Abstract: | The peptide components in neuropeptide injectio were separated with Sephadex G 25 and ACA gel filtration chromatography in this experiment. Then the liquid of different parts that were collected from gel column were identificated by UV scanning, ninhydrin, micro-- biuret reactions and thin--layer chromatography. Meanwhile, the purity and the molecular weight of the neuropeptide were determind by SDS -- Polyacrylamide gel electrophoresis, (SDS -- PAGE).The results showed that there were one main peptide component and some smaller molecular weight peptides in this injectic. The molecular weight of the main peptide component was about 14 , 400D. |
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| Keywords: | Neuropeptide Isolation and identification SDS--PAGE |
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