| 新城疫病毒中国标准强毒株F48E9株F基因的酶切分析 |
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| 引用本文： | 王莉林 张莹. 新城疫病毒中国标准强毒株F48E9株F基因的酶切分析[J]. 生物技术, 1996, 6(6): 11-13 |
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| 作者姓名： | 王莉林 张莹 |
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| 作者单位： | 哈尔滨兽医研究所兽医生物技术国家重点实验室 |
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| 摘 要： | 含新城疫病毒Ｆ４８Ｅ９株Ｆ基因的重组质粒ｐＢＦＦ经单酶切，双酶切分析，结果表明，中国的标准强毒株Ｆ４８Ｅ５株的Ｆ基因与国外的一些强毒株相比有较大的差异，多出一个ＢａｍＨＩ酶切位点，一个ＳｅａＩ酶切位点，三个ｐｓｔＩ酶切位点，少了一个ｐｓｔＩ酶切位点。
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| 关 键 词： | 新城疫病毒；F基因；酶切分析 |
Analysis of Gene of NDV F_(48)E_9Strain by Restriction Enzymes |
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| Wang Lilin, Zhang Ying, Cao DiangjunHuo Longfei, Lu Jingliang. Analysis of Gene of NDV F_(48)E_9Strain by Restriction Enzymes[J]. Biotechnology, 1996, 6(6): 11-13 |
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| Authors: | Wang Lilin Zhang Ying Cao DiangjunHuo Longfei Lu Jingliang |
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| Abstract: | The recombinant plasmid, pBFF, containing F gene of NDV F48E9 strain was mapped byrestriction enzymes. Comparing with the F genes derived from other NDV strains, a additional BamH I site, two pst I site and a Sea I site were found within the F gene encodingsequence of NDV F48E9 strain. |
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| Keywords: | NDV F genes Restriction enzymes |
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