Purification and properties of the Klebsiella aerogenes UreE metal-binding domain, a functional metallochaperone of urease |
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Authors: | Mulrooney Scott B Ward Sarah K Hausinger Robert P |
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Institution: | Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824-4320, USA. |
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Abstract: | Klebsiella aerogenes UreE, a metallochaperone that delivers nickel ions during urease activation, consists of distinct "peptide-binding" and "metal-binding" domains and a His-rich C terminus. Deletion analyses revealed that the metal-binding domain alone is sufficient to facilitate urease activation. This domain was purified and shown to exhibit metal-binding properties similar to those of UreE lacking only the His-rich tail. |
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