|
|||||
|
|
| 喉淋巴管的几种组织化学显色法比较观察 | |
| 引用本文： | 王莹,李文媛,富泽龙,刘跃光,冯克俭. 喉淋巴管的几种组织化学显色法比较观察[J]. 中国组织化学与细胞化学杂志, 2008, 17(1): 70-73. DOI: 10.3870/j.issn.1004-1850.2008.01.015 |
| 作者姓名： | 王莹 李文媛 富泽龙 刘跃光 冯克俭 |
| 作者单位： | 1. 牡丹江医学院解剖教研室 2. 牡丹江医学院附属二院五官科 3. 牡丹江肿瘤医院,黑龙江,157011 |
| 基金项目： | 黑龙江省高等学校骨干教师资助计划 |
| 摘 要： | 目的为喉毛细血管和毛细淋巴管的鉴别提供更好的技术方法。方法采用间接注射法，5′-核苷酸酶-碱性磷酸酶双重显色法（5′-Nase-ALPase），针对基底膜的免疫组化显色法。结果酶组化染色结果显示毛细血管和血管显示有清楚的蓝色轮廓，而附近的毛细淋巴管和淋巴管则呈棕色轮廓，两者易于鉴别。免疫组化染色结果显示毛细血管和血管显示清楚的棕色轮廓，而附近的毛细淋巴管和淋巴管轮廓极其浅淡，两者亦可鉴别。结论酶组化和免疫组化显色法是喉内区别毛细淋巴管和毛细血管的一种可靠且特异的方法。 |
| 关 键 词： | 组织化学显色 毛细淋巴管 喉 |
| 修稿时间： | 2007-06-12 |
COMPARISON OF SEVERAL HISTOCHEMICAL STAINING METHODS FOR LARYNX LYMPHATIC CAPILLARIES |
|
| Wang Ying,Li Wenyuan,Fu Zelong,Liu Yueguang,Feng Kejian. COMPARISON OF SEVERAL HISTOCHEMICAL STAINING METHODS FOR LARYNX LYMPHATIC CAPILLARIES[J]. Chinese Journal of Histochemistry and Cytochemistry, 2008, 17(1): 70-73. DOI: 10.3870/j.issn.1004-1850.2008.01.015 | |
| Authors: | Wang Ying Li Wenyuan Fu Zelong Liu Yueguang Feng Kejian |
| Affiliation: | Wang Ying*,Li Wenyuan1,Fu Zelong2,Liu Yueguang,Feng Kejian(Department of Anatomy,1Second Affiliated Hospital,2Mudanjiang Tumor Hospital,Mudanjiang Medical College,Mudanjiang 157011,China) |
| Abstract: | Objective To provide a better technique to distinguish lymphatic capillaries from blood capillaries in the larynx.Methods Injection technique,the 5-nucleotidas-alkaline phosphates double staining,and the immunohistochemical staining aimed at the basement membranes were utilized in this study.Results The enzyme-histochemical results showed that brown was seen in the lymphatic capillaries and lymph vessels,but blue was seen in the blood and capillaries vessels.Both were easily distinguished.The immunohistoche... |
| Keywords: | Histochemicalstaining Lymphatic capillaries larynx |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |