Complete nucleotide sequence of Tn10 |
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Authors: | Chalmers R Sewitz S Lipkow K Crellin P |
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Affiliation: | Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom. chalmers@bioch.ox.ac.uk |
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Abstract: | The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal resistance operons, and a hypothetical protein in Bacillus subtilis. The glutamate permease was fully functional when expressed, but Tn10 did not protect Escherichia coli from the toxic effects of various metals. |
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