Characterization of ten polymorphic microsatellite loci for the threatened species Grimmia curviseta Bouman (Grimmiaceae,Musci) |
| |
| Authors: | Alexandra Rodríguez-Romero Mariano Hernández Jairo Patiño Olaf Werner Juana M González-Mancebo |
| |
| Institution: | 1. Departmento de Botánica, Ecología y Fisiología Vegetal, Universidad de La Laguna, Tenerife, Spain;2. Departamento de Bioquímica, Microbiología, Biología Celular y Genética, Universidad de La Laguna, Spain;3. Instituto de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de la Laguna, Tenerife, Spain;4. Island Ecology and Evolution Research Group, Instituto de Productos Naturales y Agrobiología (IPNA-CSIC), La Laguna, Tenerife, Canary Islands, Spain;5. Departamento de Ciências Agrárias, 3cE3c, Centre for Ecology, Evolution and Environmental Changes/Azorean Biodiversity Group &6. University of Azores, Angra do Heroísmo, Azores, Portugal;7. Department of Plant Biology, University of Murcia, Murcia, Spain |
| |
| Abstract: | Grimmia curviseta Bouman is a moss species endemic to the Canary Islands in the North Atlantic Ocean, which has a very restricted distribution across the summit areas of Tenerife and La Palma islands. Using massive sequencing, we developed ten polymorphic microsatellite markers for this species. The pattern of microsatellite alleles per locus provides preliminary evidence that G. curviseta is allodiploid. The average number of alleles per locus ranged from 2 to 7, and observed heterozygosities varied from 0.022 to 1.000. A significant genetic differentiation was observed between the Tenerife and La Palma populations, suggesting that there are some limitations to dispersal. This set of microsatellites constitutes a valuable tool to investigate patterns of genetic diversity across the distribution range of G. curviseta, information that may eventually be used to establish conservation strategies for the species. Owing to the cross-amplification with the closely related species Grimmia montana Bruch & Schimp., our study also demonstrates the utility of these markers for population-level genetic analyses in the genus Grimmia. |
| |
| Keywords: | Canary Islands Conservation biology Endemism Genetic variation Microsatellite |
|
|
