Sophorolipid Production with High Yields on Whey Concentrate and Rapeseed Oil without Consumption of Lactose |
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Authors: | Hans-J. Daniel Ralf T. Otto Matthias Reuss Christoph Syldatk |
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Affiliation: | (1) Institute of Biochemical Engineering, University of Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany |
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Abstract: | Sophorolipids were produced by single-step batch cultivation of Candida bombicola ATCC 22214 on deproteinized whey concentrate and repeated feed of rapeseed oil. A mild sterilization method for whey was developed. High yields of 280 g dry sophorolipids l–1 were obtained from deproteinized whey concentrate containing 100 g lactose l–1 and 300 g rapeseed oil l–1. Surprisingly, the whey lactose was not consumed by the organism. Growth only on the oil was assumed and a high lipase activity of 24 U per g cell dry weight resulted. |
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