Histochemical method for the demonstration of the activity of phosphoglucomutase |
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Authors: | A E F H Meijer |
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Institution: | (1) Department of Pathological Anatomy, Biochemical Section, University of Amsterdam, The Netherlands |
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Abstract: | Summary A method for the demonstration of the activity of phosphoglucomutase in tissues is described. In the histochemical system the enzyme converts the substrate -D-glucose-1-phosphate to -D-glucose-6-phosphate. The resulting -D-glucose-6-phosphate is oxidized by exogenous glucose-6-phosphate dehydrogenase to D-glucono- -lactone-6-phosphate, whereby the activity of endogenous NADPH-tetrazolium oxidoreductase reduces Nitro-BT to the slightly soluble diformazan. The problems involved in the histochemical demonstration of phosphoglucomutase are discussed. |
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