外胚间充质干细胞构建组织工程骨骼肌的应用研究

目的:探讨利用大鼠颌突外胚间充质干细胞构建组织工程骨骼肌的可行性,并观察对骨骼肌缺损的修复重建的促进效应。方法:取妊娠E 11.5胎鼠颌突外胚间充质干细胞,纯化后在含5ml/L体积浓度二甲基亚砜的DMEM/F12培养基中诱导分化为骨骼肌样细胞,将细胞种植于BAM膜上培养形成组织工程骨骼肌。将其移植入大鼠骨骼肌缺损模型,手术后14 d观察骨骼肌恢复情况,同期进行组织学及免疫组化染色鉴定。结果:经诱导后外胚间充质干细胞可向骨骼肌样细胞转化,构建的组织工程骨骼肌可加速缺损的修复重建,组织学染色显示外胚间充质干细胞具有正常骨骼肌的组织形态,可表达成肌相关蛋白MyOD。结论:诱导后的外胚间充质干细胞可作为种子细胞构建组织工程骨骼肌,本实验为临床肌肉的缺损修复奠定了理论基础。

Application of Construction of Tissue Engineering Skeletal Muscle by Cultured Ectomesenchymal Stem Cell

Objective:To investigate the feasibility of constructing tissue engineering skeletal muscle with ectomesenchymal stem cells and the improvement on reconstruction of skeletal muscle defect.Methods:The ectomesenchymal stem cells were derived from E 11.5 fetal rat mandibular process,and then,these cells were differentiated into skeleton-muscle-like cells with 5 ml/L DMSO.Tissue en- gineering skeletal muscle constructed with BAM membrane and differentiated cells were transplanted to the site of muscle defect.After 14 days,all specimens were investigated with histological and immunohistochemical staining.Results:The ectomesenchymal stem cells can be differentiated to skeleton-muscle-like cells by DMSO and the tissue engineering skeletal muscle can accelerate the repair and re- construction of muscle.The tissue engineered muscles were similar to normal muscle in the characteristic of histological and morphologi- cal feature and express the positive signal of MyoD after 14 day transplantation.Conclusion:Ectomesenchymal stem cells were the ideal seeding cells in construct tissue-engineering muscle.The experimental study can provide the potential theory foundation and application value for the repair of muscle defect in the clinic.