Responses of the photosynthetic system and peroxidase activity to the rooting conditions of oak micropropagation |
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Authors: | Király István Balla Ildikó Jakab Júlia Tamás László Sárvári Éva |
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Institution: | 1.Department of Plant Physiology, E?tv?s, University, Budapest, H - 1088, Múzeum krt. 4/A, Hungary ;2.Research Institute of Fruitgrowing and Ornamentals, Budapest, H - 1223, Park u. 2, Hungary ;3.Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462, Martonvásár, Hungary ;4.Department of Plant Physiology, E?tv?s, University, Budapest, H - 1088, Múzeum krt. 4/A, Hungary ; |
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Abstract: | The effects of indole butyric acid (IBA) concentration and the duration of the plant growth regulator (PGR) treatment on the
rooting ability, peroxidase level and photosynthetic activity of young Quercus robur L. plants were studied. Four-week-old oak shoots were transplanted to rooting media supplemented with 10 or 20 μM IBA. After
2, 3, 4, 5 or 7 days the shoots were transplanted again to a fresh, PGR-free medium. On the tenth day after transfer to rooting
medium, the CO2 fixation capacity, pigment content and peroxidase activity were measured. The photosynthetic parameters varied as a function
of the time spent on medium containing PGR, showing maximum values in plants transplanted on the third to fourth day to PGR-free
medium. The rooting percentage of these plants reached its maximum within two weeks. However, peroxidase activity was the
highest in plants transferred later to PGR-free medium. The most pronounced stimulating effect on rooting was achieved with
the higher initial IBA concentration followed by a transfer to PGR-free medium on the third to fourth day. These plants showed
the highest vitality and the best rooting ability.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | oak micropropagation peroxidase photosynthetic performance root induction |
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