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Direct Force Measurement of the Interaction Between Liposome and the C2A Domain of Synaptotagmin I using Atomic Force Microscopy
Authors:Jong-Ho Park  Eun-Young Kwon  Hyo-Il Jung  Dae-Eun Kim
Affiliation:(1) Department of Mechanical Engineering, Yonsei University, Seoul, South Korea
Abstract:The binding force between a liposome and the C2A domain of synaptotagmin I was determined by an atomic force microscopy (AFM). Liposomes were immobilized on the surface of the L1 sensor chip and the C2A domains, which recognize phosphatidylserine, were chemically conjugated onto a gold-coated cantilever tip. The average interaction force between the C2A domain and the liposome was 306 (±57) pN while the force between untreated cantilever and the liposome was 58 (±16) pN. This work helps understand the physicochemical interactions between proteins and lipid vesicles for the design of high affinity protein probes against the apoptotic cell surface. Revisions requested 13 December 2005; Revisions received 9 January 2006
Keywords:atomic force microscopy (AFM)  C2A domain  force measurement  liposome  synaptotagmin I
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