Ultrastructure and cytochemistry ofPhaseolus leaf tissues infected with an isolate of tobacco necrosis virus inducing localized wilting |
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Authors: | Anna Appiano P Redolfi |
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Institution: | (1) Istituto di Fitovirologia Applicata del C.N.R., Strada delle Cacce 73, I-10135 Torino, Italy |
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Abstract: | Summary The cytopathology induced by an isolate of tobacco necrosis virus (TNV-W) causing wilted, non-self-limiting lesions inPhaseolus bean was compared with that of abiotically-induced wilting. The main cell alterations specific to TNV-W infection were ER proliferation and vesiculation, plasmolysis and plasmalemma proliferation. Later there was lysis of most cell membranes, formation of crystalline inclusions in the chloroplasts and development of fibrous structures in the cytoplasm. It is suggested that the chloroplast inclusions consist of ribulose-1,5-bisphosphate carboxylase (RuBisCo). TNV-W replicated extensively in infected cells, often forming large crystalline aggregates of virus particles. Tissue wilting, as well as crystallization of the virus and RuBisCo, may have been caused by cell dehydration due to the loss of plasmalemma integrity.Abbreviations CA
chromic acid
- CI
crystalline inclusion
- DG
dense granule
- E
ethanol
- ER
endoplasmic reticulum
- FS
fibrous structure
- H2O2
hydrogen peroxyde
- MVB
multivesicular body
- Pb
lead citrate
- PTA
phosphotungstic acid
- RuBisCo
ribulose-1,5-bisphosphate carboxylase
- TNV-W
tobacco necrosis virus-wilting
- UA
uranyl acetate |
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Keywords: | Cytopathology Tobacco necrosis virus Bean Abiotic stress RuBisCo |
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