| 新疆泥火山细菌群落PCR-SSCP 分析 |
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| 引用本文： | 路盼盼,李建辉,张亚平. 新疆泥火山细菌群落PCR-SSCP 分析[J]. 微生物学通报, 2011, 38(2): 195-198 |
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| 作者姓名： | 路盼盼 李建辉 张亚平 |
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| 作者单位： | 石河子大学生命科学学院,新疆石河子,832003 |
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| 基金项目： | 国家自然科学基金项目(No. 30860001) |
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| 摘 要： | 采用单链构象多态性(SSCP)技术, 以16S rDNA 基因的V3 区为靶对象, 分析泥火山细菌多样性及其群落结构。通过对泥火山不同月份的不同深度土样DNA 提取后, 针对16S rDNA 进行PCR 扩增出236 bp 大小片段, 通过SSCP 电泳对泥火山细菌进行季节性多样性分析, 并对主要条带进行克隆分析。结果显示: 新疆泥火山细菌不同季节多态性明显, 而且这种多态性容易受生态和气候的影响; 假单胞菌属是泥火山土壤优势菌群, 在泥火山区土壤中广泛分布, 受生态和气候环境影响较小。
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| 关 键 词： | 泥火山 PCR-SSCP 16S rDNA |
| 收稿时间： | 2010-09-07 |
| 修稿时间： | 2010-11-23 |
PCR-SSCP analysis bacterial community of Mud Volcano in Xinjiang |
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| LU Pan-Pan,LI Jian-Hui and ZHANG Ya-Ping. PCR-SSCP analysis bacterial community of Mud Volcano in Xinjiang[J]. Microbiology China, 2011, 38(2): 195-198 |
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| Authors: | LU Pan-Pan LI Jian-Hui ZHANG Ya-Ping |
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| Affiliation: | College of Life Science, Shihezi University, Shihezi, Xinjiang 832003, China;College of Life Science, Shihezi University, Shihezi, Xinjiang 832003, China;College of Life Science, Shihezi University, Shihezi, Xinjiang 832003, China |
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| Abstract: | A technology sigle-strand conformation polymorphism (SSCP) and a target 16S rDNA gene on V3 region were used to analyze mud volcano microbial community and structure. After extracting DNA samples from different mud deepth and month, get a 236 bp fragent by PCR. The fragment was used to analyze the microorganisms seasonal diversity of mud volcano by SSCP, and some target feagments were analyzed clone. Resluts showed: the bacteria polymorphism in Xijiang mud volcano soil is significant, which is vulnerable to ecological and climatics factors. Pseudomonas is a dominant microorganism in Xijiang mud volcano, it distributes widely in mud volcano and was affected little by ecological and climatics. |
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| Keywords: | Mud volcano PCR-SSCP 16S rDNA |
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