| 紫外辐射诱发NIH3T3细胞凋亡时DNA断裂的特性 |
| |
| 引用本文： | 胡庆柳,丁振华,谭小华,王红.紫外辐射诱发NIH3T3细胞凋亡时DNA断裂的特性[J].生物化学与生物物理进展,1998,25(1):53-56. |
| |
| 作者姓名： | 胡庆柳 丁振华 谭小华 王红 |
| |
| 作者单位： | 第一军医大学放射医学教研室，广州 510515;第一军医大学放射医学教研室，广州 510515;南方医院消化科，广州 510515;第一军医大学放射医学教研室，广州 510515 |
| |
| 基金项目： | 国家自然科学基金资助项目(39670192). |
| |
| 摘 要： | 用常规琼脂糖凝胶电泳UVB照射后培养不同时间的NIH3T3细胞DNA，两个样本均未出现凋亡梯形带，而用相同条件处理的昆明小鼠胸腺细胞DNA出现了典型的梯形带.再用反转电场琼脂糖凝胶电泳(FIGE)UVB照射后培养不同时间的NIH3T3细胞DNA，发现DNA先断裂成低于23 kb的大分子片段，然后进一步断裂成小分子片段，但始终未出现梯形带，说明DNA并不总是从核小体之间断裂的.
|
| 关 键 词： | 细胞凋亡 DNA梯形带 紫外辐射 |
| 收稿时间： | 1996/11/20 0:00:00 |
| 修稿时间： | 1997/2/28 0:00:00 |
The Characteristics of DNA Cleavage of Apoptotic NIH3T3 Cells Induced by Ultraviolet Irradiation |
| |
| HU Qing-liu,DING Zhen-hu,TAN Xiao-hua and WANG Hong.The Characteristics of DNA Cleavage of Apoptotic NIH3T3 Cells Induced by Ultraviolet Irradiation[J].Progress In Biochemistry and Biophysics,1998,25(1):53-56. |
| |
| Authors: | HU Qing-liu DING Zhen-hu TAN Xiao-hua and WANG Hong |
| |
| Institution: | Department of Radiation Medicine, The First Military Medical University, Guangzhou 510515, China;Department of Radiation Medicine, The First Military Medical University, Guangzhou 510515, China;Department of Epidemiology, Nanfang Hospital, Guangzhou 510515, China;Department of Radiation Medicine, The First Military Medical University, Guangzhou 510515, China |
| |
| Abstract: | When conventional gel electrophoresis was used, it was found that DNA of NIH3T3 cells cultured for different time after UVB irradiation had no DNA ladders, but thymocytes of Kunming mouse processed by the same method had DNA ladder. The results of field inversion electrophoresis showed that after UVB irradiation, the DNA of NIH3T3 cleaved into high molecular weight fragments at first, and then into low molecular weight fragments, still without the appearance of DNA ladders. It is suggested that DNA cleavage of apoptotic cell is not always initiate at internucleosome. |
| |
| Keywords: | apoptosis DNA ladder ultraviolet irradiation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
