Structure of carbohydrate chains of fucolectin from the bark of golden rain shrub Laburnum anagyroides

A reductive LiBH4-ButOH cleavage of N-glycosylamide carbohydrate-peptide bond allowed splitting off of oligosaccharide chains of the fucolectin, the bark agglutinin from the shrub golden rain Laburnum anagyroides (LABA). Four N-glycans were isolated by HPLC, and their structures were elucidated by monosaccharide analysis and 1H NMR (500 MHz) spectroscopy: Man2Fuc1XyllGlcNAc2 (M2FX), Man3XyllGlcNAc2 (M3X), Man3FuclXyllGlcNAc2 (M3FX), and Man3XyllFucIGlcNAc3 (NM3FX). All the N-glycans contain D-xylose and three of them, L-fucose; they were found to be in a 1 : 8 : 1 : 3 ratio.