Expression and secretion of human apolipoprotein A-I in the heart |
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Authors: | Baroukh Nadine Lopez Camilo E Saleh Maria Carla Recalde Delia Vergnes Laurent Ostos Maria A Fiette Laurence Fruchart Jean-Charles Castro Graciela Zakin Mario M Ochoa Alberto |
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Affiliation: | Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India. sampa@cmb2.saha.ernet.in |
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Abstract: | Nuclear envelope-peripheral heterochromatin fractions contain multiple histone kinase activities. In vitro assays and amino-terminal sequencing show that one of these activities co-isolates with heterochromatin protein 1 (HP1) and phosphorylates histone H3 at threonine 3. Antibodies recognizing this post-translational modification reveal that in vivo phosphorylation at threonine 3 commences at early prophase in the vicinity of the nuclear envelope, spreads to pericentromeric chromatin during prometaphase and is fully reversed by late anaphase. This spatio-temporal pattern is distinct from H3 phosphorylation at serine 10, which also occurs during cell division, suggesting segregation of differentially phosphorylated chromatin to different regions of mitotic chromosomes. |
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Keywords: | Author Keywords: Histone Phosphorylation Heterochromatin protein 1 |
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