Human catalytic antibody Se-scFv-B3 with high glutathione peroxidase activity |
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Authors: | Huo Rui Wei Jingyan Xu Junjie Lv Shaowu Zheng Qingchuan Yan Fei Su Jiaming Fan Jia Li Jieshuai Duan Yujing Yu Yang Jin Fenghai Sun Weiguo Shi Yi Cong Dengli Li Wei Yan Ganglin Luo Guimin |
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Affiliation: | College of Pharmaceutical Science, Jilin University, Changchun 130021, P.R. China. |
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Abstract: | In order to generate catalytic antibodies with glutathione peroxidase (GPX) activity, we prepared GSH-S-2,4-dinitrophenyl t-butyl ester (GSH-S-DNPBu) as target antigen. Three clones (A11, B3, and D5) that bound specifically to the antigen were selected from the phage display antibody library (human synthetic VH + VL single-chain Fv fragment (scFv) library). Analysis of PCR products using gel electrophoresis and sequencing showed that only clone B3 beared intact scFv-encoding gene, which was cloned into the expression vector pPELB and expressed as soluble form (scFv-B3) in Escherichia coli Rosetta. The scFv-B3 was purified by Ni(2+)-immobilized metal affinity chromatography (IMAC). The yield of purified proteins was about 2.0-3.0 mg of proteins from 1 L culture. After the active site serines of scFv-B3 were converted into selenocysteines (Secs) with the chemical modification method, we obtained the human catalytic antibody (Se-scFv-B3) with GPX activity of 1288 U/micromol. Copyright (c) 2008 John Wiley & Sons, Ltd. |
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Keywords: | glutathione peroxidase (GPX) catalytic antibody single‐chain Fv fragment (scFv) antibody library chemical modification selenium |
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