Cellular events in tolerance IX: Maintenance of immunological tolerance in the presence of normal B-cell precursors and in the absence of demonstrable suppression |
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Authors: | PS Pillai David W Scott |
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Institution: | Department of Microbiology and Immunology, Division of Immunology, Box 3010, Duke University Medical Center, Durham, North Carolina 27710 U.S.A. |
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Abstract: | The cellular events involved in immunological tolerance to fluoresceinated sheep gammaglobulin (FL-SGG) were analyzed at the level of hapten-specific B cells. One single iv injection of FL-SGG induced tolerance as measured by challenge with thymus-dependent (FL-KLH) or thymus-independent (FL-Ficoll) antigens in vivo or thymus-independent (FL-LPS) antigen in vitro. As noted earlier, unresponsiveness was maintained until 6–8 weeks after tolerance induction. Limiting-dilution precursor analysis demonstrated a reduction in B-cell precursors on Day 7 after tolerogen treatment; precursor frequencies returned to control levels by 3–4 weeks. This recovery of precursors in the presence of stable tolerance was not due to suppressor activity. Rather, results show that tolerant hapten-specific B cells are clonally anergic and display a reduced burst size in response to antigen. Hence, unresponsiveness is maintained in the presence of apparently normal precursor levels by an intrinsic defect in antigen-specific B cells. |
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Keywords: | To whom all correspondence should be addressed |
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