|
|||||
|
|
| 缓激肽抑制大鼠DRG分离神经元的GABA激活电流 | |
| 作者姓名： | Lu CY Si JQ Li ZW |
| 作者单位： | 1. 湖北省郧阳医学院生理教研室 2. 新疆石河子医学院生理教研室 3. 同济医科大学实验医学研究中心,武汉,430030 |
| 基金项目： | 国家自然科学基金!39370239 |
| 摘 要： | 本文应用全细胞膜片箝技术在新鲜分离的大鼠背根神经节（ＤＲＧ）神经元上研究缓激肽（ＢＫ）对γ－氨基丁酸（ＧＡＢＡ）反应的调制作用。结果发现：在３４个对ＧＡＢＡ反应的细胞中有３１个细胞对ＢＫ敏感。在对ＢＫ敏感并引起内向电流的２７个细胞中预加ＢＫ,对ＧＡＢＡ－激活电流具有明显的抑制作用,如１０＾－６ｍｏｌ／Ｌ的ＢＫ可抑制ＧＡＢＡ（１０＾－４ｍｏｌ／Ｌ）激活电流３０％。ＢＫ可将ＧＡＢＡ量效曲线明显下移,并 |
| 关 键 词： | 缓激肽 背根神经节 全细胞膜片箝 GABA |
| 修稿时间： | 1997年1月11日 |
Inhibition of GABA-activated currents in isolated rat DRG neurons by bradykinin |
|
| Lu CY,Si JQ,Li ZW.Inhibition of GABA-activated currents in isolated rat DRG neurons by bradykinin[J].Acta Physiologica Sinica,1998,50(4):373-378. | |
| Authors: | Lu C Y Si J Q Li Z W |
| Institution: | Research Center of Experimental Medicine, Tongji Medical University, Wuhan 430030. |
| Abstract: | In the present paper, the modulation of GABA-activated currents by bradykinin (BK) was studied in DRG neurons freshly isolated from rat using whole-cell patch-clamp techniques. Among the neurons (n = 34) responding to GABA, 27 were sensitive to BK. Pre-application of BK, eliciting an inward current alone, inhibited GABA-activated current markedly. For example, 10(-6) mol/L BK suppressed GABA-activated current by about 30%. BK shifted the GABA dose-response curve downward obviously and depressed the maximal response to GABA by 1/3 or more while the Kd value was unchanged. The results suggest that BK inhibits GABA-activated current noncompetitively in rat DRG neurons. |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |