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Multiple-contrast X-ray micro-CT visualization of colon malformations and tumours in situ in living mice
Authors:Choquet Philippe  Calon Alexandre  Breton Elodie  Beck Felix  Domon-Dell Claire  Freund Jean-Noël  Constantinesco André
Affiliation:Service de biophysique et de médecine nucléaire, h?pitaux universitaires de Strasbourg, h?pital de Hautepierre, 1, avenue Molière, 67098 Strasbourg, France.
Abstract:The development of new therapeutic approaches against colorectal cancer requires preclinical studies in mice. In vivo imaging could greatly facilitate these trials, but the small size of the animals is a major limitation for the direct visualization of intestinal tissue. Here we report a method of in vivo imaging of the mouse intestine based on X-ray micro-computed tomography using multiple contrast agents. This method was validated in the model of non-cancerous polyp-like heteroplasia that spontaneously develops in the caecum area of Cdx2+/- mutant mice and in the model of colon adenocarcinoma induced by administration of the chemical carcinogen azoxymethane. As a simple and non-invasive method, multiple-contrast X-ray micro-computed tomography is appropriate for pre-clinical studies of intestinal diseases in living mice.
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