Multiple-contrast X-ray micro-CT visualization of colon malformations and tumours in situ in living mice |
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Authors: | Choquet Philippe Calon Alexandre Breton Elodie Beck Felix Domon-Dell Claire Freund Jean-Noël Constantinesco André |
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Affiliation: | Service de biophysique et de médecine nucléaire, h?pitaux universitaires de Strasbourg, h?pital de Hautepierre, 1, avenue Molière, 67098 Strasbourg, France. |
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Abstract: | The development of new therapeutic approaches against colorectal cancer requires preclinical studies in mice. In vivo imaging could greatly facilitate these trials, but the small size of the animals is a major limitation for the direct visualization of intestinal tissue. Here we report a method of in vivo imaging of the mouse intestine based on X-ray micro-computed tomography using multiple contrast agents. This method was validated in the model of non-cancerous polyp-like heteroplasia that spontaneously develops in the caecum area of Cdx2+/- mutant mice and in the model of colon adenocarcinoma induced by administration of the chemical carcinogen azoxymethane. As a simple and non-invasive method, multiple-contrast X-ray micro-computed tomography is appropriate for pre-clinical studies of intestinal diseases in living mice. |
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