Kinetic and electrophoretic abnormality of cyclic AMP phosphodiesterase in genetically obese mouse adipocytes |
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| Authors: | J C Kaplan A L Pichard M H Laudat P Laudat |
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| Affiliation: | 1. Institut de Pathologie Moléculaire 75014 — Paris, France;2. Unité de Recherches sur le Métabolisme des Lipides Faculté de Médecine Cochin 75014 — Paris, France |
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| Abstract: | In the soluble fraction of adipocytes from Bar Harbor ob/ob mice, phosphodiesterase exhibits abnormal kinetics for cyclic AMP as compared to adipocyte extracts from normal littermates: a 10 fold increase of the high Km enzyme and a 2 fold increase of the corresponding Vmax. Upon starch gel electrophoresis an abnormal pattern was seen. These abnormalities were found regardless the nutritional status of the animals. There was no kinetic abnormality with cyclic GMP. |
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