Effect of sugars,amino acids,and culture technique on maturation of somatic embryos of Pinus strobus on medium with two gellan gum concentrations |
| |
Authors: | Garin Élisabeth Bernier-Cardou Michèle Isabel Nathalie Klimaszewska Krystyna Plourde Ariane |
| |
Institution: | (1) Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, P.O. Box 3800, 1055 du P.E.P.S., Sainte-Foy, Québec, Canada, G1V 4C7;(2) Present address: Centre de recherche en biologie forestière, Pavillon C.-E. Marchand, Université Laval, Sainte-Foy, Québec, Canada, G1K 7P4 |
| |
Abstract: | Maturation of five embryogenic lines of Pinus strobus L. was tested on media with various sugars and sources of organic nitrogen, and solidified with two gellan gum concentrations
(0.6 and 1.0%). Mature somatic embryo production was more abundant at 1.0% gellan gum than at 0.6%. Complex combinations of
amino acids had little effect on mature embryo production of most tested embryogenic lines. Increasing glutamine concentration
of the maturation medium from 1.7 to 7.3 g l−1 was beneficial to one embryogenic line. Increasing sucrose concentration or substituting part of the sucrose with mannitol
or sorbitol had variable effects on somatic embryo maturation depending on the embryogenic line. A medium with 88 mM sucrose
plus 175 mM sorbitol solidified with 1.0% gellan gum produced high numbers of somatic embryos in four out of five embryogenic
lines tested.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | carbohydrates eastern white pine organic nitrogen somatic embryogenesis |
本文献已被 SpringerLink 等数据库收录! |
|